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pmcherry atg5  (Addgene inc)


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    Addgene inc pmcherry atg5
    Pmcherry Atg5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmcherry+atg5+13095+plasmids/pmCherry-ATG5+(Plasmid+%2313095)/pmc12607867-1-0-2
    Average 93 stars, based on 24 article reviews
    pmcherry atg5 - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy
    Article Snippet: pMXs-IP HA-PARK2 (38248), YFP-PARK2 (23955), pRK5-MYC-PARK2 (17612), mCherry-PARK2 (23956), pCDNA-DEST47 PINK1-C-GFP (13316), optineurin(OPTN)-EGFP (27052) and pmCherry-ATG5 (13095) plasmids were purchased from Addgene.



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    (A) Schematic of autophagosome biogenesis pathway, highlighting the elongation complex. (B-C) Representative micrographs of DRG neurons transiently expressing <t>mCh-ATG5</t> from female (B) and male (C) young adult mice. Arrows indicate mCh-ATG5 puncta. Scale bars, 2 μm. (D) Quantification of the rate of mCh-ATG5 puncta formation in live-cell imaging of DRG neurons (median ± quartiles; n ≥ 22 neurons from three biological replicates of each sex). ns, not significant by unpaired t tests (p > 0.39). (E) Immunoblots of DRG lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (F-G) Quantification of protein levels of immunoblots in (E), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant by two-way ANOVA with Šídák’s multiple comparisons test. (H) Immunoblots of brain lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (I-J) Quantification of protein levels of immunoblots in (H), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant; **p < 0.005 by two-way ANOVA with Šídák’s multiple comparisons test.
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    pmcherry atg5 13095 plasmids  (Addgene inc)
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    (A) Schematic of autophagosome biogenesis pathway, highlighting the elongation complex. (B-C) Representative micrographs of DRG neurons transiently expressing <t>mCh-ATG5</t> from female (B) and male (C) young adult mice. Arrows indicate mCh-ATG5 puncta. Scale bars, 2 μm. (D) Quantification of the rate of mCh-ATG5 puncta formation in live-cell imaging of DRG neurons (median ± quartiles; n ≥ 22 neurons from three biological replicates of each sex). ns, not significant by unpaired t tests (p > 0.39). (E) Immunoblots of DRG lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (F-G) Quantification of protein levels of immunoblots in (E), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant by two-way ANOVA with Šídák’s multiple comparisons test. (H) Immunoblots of brain lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (I-J) Quantification of protein levels of immunoblots in (H), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant; **p < 0.005 by two-way ANOVA with Šídák’s multiple comparisons test.
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    Image Search Results


    (A) Schematic of autophagosome biogenesis pathway, highlighting the elongation complex. (B-C) Representative micrographs of DRG neurons transiently expressing mCh-ATG5 from female (B) and male (C) young adult mice. Arrows indicate mCh-ATG5 puncta. Scale bars, 2 μm. (D) Quantification of the rate of mCh-ATG5 puncta formation in live-cell imaging of DRG neurons (median ± quartiles; n ≥ 22 neurons from three biological replicates of each sex). ns, not significant by unpaired t tests (p > 0.39). (E) Immunoblots of DRG lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (F-G) Quantification of protein levels of immunoblots in (E), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant by two-way ANOVA with Šídák’s multiple comparisons test. (H) Immunoblots of brain lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (I-J) Quantification of protein levels of immunoblots in (H), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant; **p < 0.005 by two-way ANOVA with Šídák’s multiple comparisons test.

    Journal: bioRxiv

    Article Title: Sex does not Influence Neuronal Autophagy throughout Aging in Mice

    doi: 10.1101/2025.01.16.633428

    Figure Lengend Snippet: (A) Schematic of autophagosome biogenesis pathway, highlighting the elongation complex. (B-C) Representative micrographs of DRG neurons transiently expressing mCh-ATG5 from female (B) and male (C) young adult mice. Arrows indicate mCh-ATG5 puncta. Scale bars, 2 μm. (D) Quantification of the rate of mCh-ATG5 puncta formation in live-cell imaging of DRG neurons (median ± quartiles; n ≥ 22 neurons from three biological replicates of each sex). ns, not significant by unpaired t tests (p > 0.39). (E) Immunoblots of DRG lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (F-G) Quantification of protein levels of immunoblots in (E), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant by two-way ANOVA with Šídák’s multiple comparisons test. (H) Immunoblots of brain lysates from young, young adult, aged, and advanced aged mice of both sexes (n= 3 biological replicates for each sex at each age. Total protein was used as a loading control; normalization factor is indicated below each blot as a percentage. (I-J) Quantification of protein levels of immunoblots in (H), normalized to total protein and represented as a fold change relative to the mean for lysates from young mice (mean ± sem). ns, not significant; **p < 0.005 by two-way ANOVA with Šídák’s multiple comparisons test.

    Article Snippet: Constructs used include: mCherry-ATG13 (subcloned from Addgene, 22875; deposited by Noboru Mizushima), mCherry-ATG5 (Addgene 13095), Halo-ATG14L (subcloned from Addgene, 21635; deposited by Tamotsu Yoshimori), SNAP-WIPI1A (subcloned from Addgene 38272), Halo-DFCP1 (subcloned from Addgene 38269), and Halo-WIPI2B (subcloned from GFP-WIPI2B ( )), all previously published in ( ; ).

    Techniques: Expressing, Live Cell Imaging, Western Blot, Control

    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport and Clearance

    doi: 10.1016/j.devcel.2020.03.011

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: pmCherry-ATG5 , Gift from Roberta Gottlieb (Hamacher-Brady et al., 2007) , Addgene plasmid # 13095; http://n2t.net/addgene:13095 ; RRID: Addgene_13095.

    Techniques: Ubiquitin Proteomics, Recombinant, Protease Inhibitor, Mutagenesis, shRNA, Plasmid Preparation, Software